human mouse rat hdls  (Biorbyt)

Journal: Journal of Neuroscience Research

Article Title: Neuronal extracellular microRNAs miR‐124 and miR‐9 mediate cell–cell communication between neurons and microglia

doi: 10.1002/jnr.24344

Figure Lengend Snippet: Antibody characterization

Article Snippet: Rabbit polyclonal antibodies for human/mouse/rat HDLs (immunogen: KLH conjugated synthetic peptide from a 41–125 amino acid sequence of human HDL) were purchased from Biorbyt (cat#orb1003; 0.5 mg/ml, dilution 1:1,000; RRID: AB_2737031).

Techniques: Concentration Assay, Marker, Binding Assay, Immunofluorescence, Blocking Assay, Sequencing, Western Blot

Journal: Journal of Neuroscience Research

Article Title: Neuronal extracellular microRNAs miR‐124 and miR‐9 mediate cell–cell communication between neurons and microglia

doi: 10.1002/jnr.24344

Figure Lengend Snippet: Antibody characterization

Article Snippet: Rabbit polyclonal antibodies for human/mouse/rat HDLs , KLH conjugated synthetic peptide from a 41–125 amino acid sequence of human HDL (human, mouse, rat) , Biorbyt, cat#orb1003, RRID: AB_2737031 , control: absence of non‐specific bands on the western blot , 0.5 mg/ml, dilution 1:1,000.

Techniques: Control, Concentration Assay, Marker, Binding Assay, Immunofluorescence, Bioprocessing, Blocking Assay, Sequencing, Western Blot

Journal: Journal of Neuroscience Research

Article Title: Neuronal extracellular microRNAs miR‐124 and miR‐9 mediate cell–cell communication between neurons and microglia

doi: 10.1002/jnr.24344

Figure Lengend Snippet: Analysis of the level of expression of neuronal microRNAs miR‐124 and miR‐9 in HDL complexes. (a) Western blot analysis of the expression of 40‐kDa protein from HDL complexes in high molecular weight (>100 kDa) versus low molecular weight (10–100 kDa) fractions of BSCM. Ponseus S staining was used as a protein loading control. (b) Immunoprecipitation of HDL with miR‐124 and miR‐9. Anti‐HDL antibodies were adsorbed to plastic and >100‐kDa or 10–100‐kDa fractions of BSCM were added to antibodies as described in Materials and Methods . Expression of miR‐124 was assessed in HDLs bound to anti‐HDL antibodies (>100 kDa:HDL and 10–100 kDa:HDL) and in solution unbound to HDL in the >100 and 10–100‐kDa fractions (>100 and 10–100 kDa). (c, d) Comparison of expression of miR‐124 (c) miR‐9 (d) in untreated BSCM versus BSCM treated with RNase (fractions of >100 and 10–100 kDa). In (b–d), mean ± SD of three separate experiments is shown on dotplots ( n = 3; ** p < 0.01; *** p < 0.001; **** p < 0.0001; **** p < 0.00001; unpaired Student's t test) [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Rabbit polyclonal antibodies for human/mouse/rat HDLs , KLH conjugated synthetic peptide from a 41–125 amino acid sequence of human HDL (human, mouse, rat) , Biorbyt, cat#orb1003, RRID: AB_2737031 , control: absence of non‐specific bands on the western blot , 0.5 mg/ml, dilution 1:1,000.

Techniques: Expressing, Western Blot, High Molecular Weight, Molecular Weight, Staining, Control, Immunoprecipitation, Comparison

Journal: Journal of Neuroscience Research

Article Title: Neuronal extracellular microRNAs miR‐124 and miR‐9 mediate cell–cell communication between neurons and microglia

doi: 10.1002/jnr.24344

Figure Lengend Snippet: Model of horizontal transfer of miR‐124 and miR‐9 from neurons to microglia. Electrically active neurons secrete exosomes with miR‐9, as well as miR‐124 with miR‐9 in naked form and/or in complex with low molecular weight proteins. Astrocytes secrete HDLs that bind to miR‐124 and miR‐9 in the extracellular space. Exosomes with miR‐9, miR‐124:HDL, and miR‐9:HDL complexes are taken up by microglia. MiR‐9 and miR‐124 are translocated to the cytoplasm of microglia via fusion of miR‐9‐positive exosomes with plasma membrane or transport of HDL‐miR‐124 and HDL‐miR‐9 into the cytoplasm via scavenger receptors. In the cytoplasm, miR‐124 (and possibly miR‐9) deactivate microglia, leading to downregulation of MHC class II and CD45. Inside the cell, miR‐124 are known to inhibit the CEBPα‐PU.1 pathway, whereas miR‐9 inhibit NFκB. Both of these pathways are important for macrophage/microglia activation and expression of activation markers such as MHC class II and CD45

Article Snippet: Rabbit polyclonal antibodies for human/mouse/rat HDLs , KLH conjugated synthetic peptide from a 41–125 amino acid sequence of human HDL (human, mouse, rat) , Biorbyt, cat#orb1003, RRID: AB_2737031 , control: absence of non‐specific bands on the western blot , 0.5 mg/ml, dilution 1:1,000.

Techniques: Molecular Weight, Clinical Proteomics, Membrane, Activation Assay, Expressing